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Items 1 to 10 of about 5827
5. Liu C, Cao Z, He S, Sun Z, Chen W: The effects and mechanism of phycocyanin removal from water by high-frequency ultrasound treatment. Ultrason Sonochem; 2018 Mar;41:303-309
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  • [Source] The source of this record is MEDLINE®, a database of the U.S. National Library of Medicine.
  • [Title] The effects and mechanism of phycocyanin removal from water by high-frequency ultrasound treatment.
  • The effects and mechanism of phycocyanin removal from water by high-frequency ultrasound treatment were studied.
  • The efficiency of sonication treatment in removing proteins derived from algal cells was investigated, and the factors influencing the process, including the effects of coagulation, were also studied.
  • In addition, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), the three-dimensional fluorescence spectrum, and mass spectrum were used to illustrate the removal mechanism.
  • The results indicated that phycocyanin can be degraded to the point where it is barely detectable in water samples after 180min of high-frequency sonication.
  • While the total nitrogen (TN) concentration remained consistent during the entire sonication process (240min), about 78.9% of the dissolved organic nitrogen (DON) was oxidized into inorganic nitrogen.
  • The sonication effect was greatly influenced by the ultrasound frequency, with 200kHz having the highest removal performance due to the large production of hydroxyl (HO) radicals.
  • Coagulation was adversely influenced by sonication in the first 60min due to the cross-linking reaction between protein molecules caused by the sonication.
  • The influence of sonication weakened with sonication time due to the further degradation of the proteins by ultrasound.
  • The variation of the TN, DON, and inorganic nitrogen indicated that the main mechanism occurring during the high-frequency sonication of the phycocyanin was the direct oxidation of the radicals, which was totally different from of the mechanism occurring during ultrasound with low frequency.

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  • [Copyright] Copyright © 2017 Elsevier B.V. All rights reserved.
  • (PMID = 29137756.001).
  • [ISSN] 1873-2828
  • [Journal-full-title] Ultrasonics sonochemistry
  • [ISO-abbreviation] Ultrason Sonochem
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] Netherlands
  • [Keywords] NOTNLM ; Drinking water / High-frequency / Phycocyanin / Protein / Ultrasound
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6. Tang P, Chen Z: Capillary electrochromatography using knitted aromatic polymer as the stationary phase for the separation of small biomolecules and drugs. Talanta; 2018 Feb 01;178:650-655
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  • [Source] The source of this record is MEDLINE®, a database of the U.S. National Library of Medicine.
  • [Title] Capillary electrochromatography using knitted aromatic polymer as the stationary phase for the separation of small biomolecules and drugs.
  • Hypercrosslinked polymers (HCPs) are currently receiving great attention due to their unique characteristics and potential uses in diverse areas.
  • However, the field of HCPs for open-tubular capillary electrochromatography (OT-CEC) separations has not been explored.
  • Here, a knitted aromatic polymer (KAP) was in-situ grown on the inner wall of the capillary column for OT-CEC for the first time.
  • The silylating reagent containing phenyl was served as monomer, immobilized on the inner wall of the capillary column, and then KAPs-modified capillary column was prepared through in-situ hypercrosslinking reaction.
  • The surface structure and morphology of KAPs-modified capillary column was characterized by Fourier transform infrared spectra (FT-IR) and scanning electron microscopy (SEM).
  • The prepared capillary columns showed good separation performance for neutral compounds, small biomolecules, such as nucleosides, amino acids, small peptides, and non-steroidal anti-inflammatory drugs, sulfa drugs.
  • In addition, the KAPs-modified capillaries showed good reproducibility, with relative standard deviations for intra-day, inter-day and column-to-column runs less than 1.59%, 2.55%, and 5.19% respectively.
  • The strategy of in-situ immobilization of KAPs provides a new approach for the application of the material in the analytical fields.

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  • [Copyright] Copyright © 2017 Elsevier B.V. All rights reserved.
  • (PMID = 29136876.001).
  • [ISSN] 1873-3573
  • [Journal-full-title] Talanta
  • [ISO-abbreviation] Talanta
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] Netherlands
  • [Keywords] NOTNLM ; Amino acids / Capillary electrochromatography / Knitted aromatic polymer / Non-steroidal anti-inflammatory drugs / Nucleosides
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7. Li B, Takahashi D, Kawamura Y, Uemura M: Plasma Membrane Proteomics of Arabidopsis Suspension-Cultured Cells Associated with Growth Phase Using Nano-LC-MS/MS. Methods Mol Biol; 2018;1696:185-194
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  • [Source] The source of this record is MEDLINE®, a database of the U.S. National Library of Medicine.
  • [Title] Plasma Membrane Proteomics of Arabidopsis Suspension-Cultured Cells Associated with Growth Phase Using Nano-LC-MS/MS.
  • Arabidopsis thaliana suspension-cultured cells (T87 line) are important model system for studies of responses to biotic and abiotic stresses at the cellular level in vitro since the cells have certain advantages compared with the whole plant system.
  • However, the physiological and morphological characteristics of the cells are influenced by the progress of the growth phase of cells, which may result in different stress tolerance.
  • To obtain comprehensive proteome profiles of the plasma membrane of Arabidopsis thaliana T87 suspension-cultured cells at the lag, log, or stationary growth phase, a shotgun proteomics method using nano-LC-MS/MS is used.
  • The results obtained indicate that proteome profiles of the plasma membrane with the progress of the growth phase of cells dynamically changed, which may be associated with the physiological and morphological characteristics of the plasma membrane of the suspension-cultured cells.
  • The proteomics results are further applied to explain different responsive patterns in the plasma membrane to cold acclimation and ABA treatment, which lead to understanding of different freezing tolerance associated with the growth phase of the cells.

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  • (PMID = 29086404.001).
  • [ISSN] 1940-6029
  • [Journal-full-title] Methods in molecular biology (Clifton, N.J.)
  • [ISO-abbreviation] Methods Mol. Biol.
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] United States
  • [Keywords] NOTNLM ; Culture cells / Growth phase / Nano-LC-MS/MS / Plasma membrane / Proteomics
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8. Saragadam T, Punekar NS: Novel Route for Agmatine Catabolism in Aspergillus niger: 4-Guanidinobutyrase Assay. Methods Mol Biol; 2018;1694:163-172
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  • [Source] The source of this record is MEDLINE®, a database of the U.S. National Library of Medicine.
  • [Title] Novel Route for Agmatine Catabolism in Aspergillus niger: 4-Guanidinobutyrase Assay.
  • The enzyme 4-guanidinobutyrase (GBase) catalyzes the hydrolysis of 4-guanidinobutyric acid (GB) to 4-aminobutyric acid (GABA) and urea.
  • Here we describe methods to estimate urea and GABA that were suitably adapted from the published literature.
  • The urea is determined by colorimetric assay using modified Archibald's method.
  • However, the low sensitivity of this method often renders it impractical to perform fine kinetic analysis.
  • To overcome this limitation, a high sensitive method for detecting GABA is exploited that can even detect 1 μM of GABA in the assay mixture.
  • The samples are deproteinized by perchloric acid (PCA) and potassium hydroxide treatment prior to HPLC analysis of GABA.
  • The method involves a pre-column derivatization with o-phthalaldehyde (OPA) in combination with the thiol 3-mercaptopropionic acid (MPA).
  • The fluorescent GABA derivative is then detected after reversed phase high performance liquid chromatography (RP-HPLC) using isocratic elution.
  • The protocols described here are broadly applicable to other biological samples involving urea and GABA as metabolites.

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  • (PMID = 29080167.001).
  • [ISSN] 1940-6029
  • [Journal-full-title] Methods in molecular biology (Clifton, N.J.)
  • [ISO-abbreviation] Methods Mol. Biol.
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] United States
  • [Keywords] NOTNLM ; 3-Mercaptopropionic acid / 4-Guanidinobutyrase / 4-Guanidinobutyric acid / Agmatine / GABA / Isocratic / Reversed phase HPLC / Urea / o-Phthalaldehyde
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9. Yan H, Song X, Tian K, Chen Y, Xiong Y, Min S: Quantitative determination of additive Chlorantraniliprole in Abamectin preparation: Investigation of bootstrapping soft shrinkage approach by mid-infrared spectroscopy. Spectrochim Acta A Mol Biomol Spectrosc; 2017 Sep 01;191:296-302
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  • [Source] The source of this record is MEDLINE®, a database of the U.S. National Library of Medicine.
  • [Title] Quantitative determination of additive Chlorantraniliprole in Abamectin preparation: Investigation of bootstrapping soft shrinkage approach by mid-infrared spectroscopy.
  • A novel method, mid-infrared (MIR) spectroscopy, which enables the determination of Chlorantraniliprole in Abamectin within minutes, is proposed.
  • We further evaluate the prediction ability of four wavelength selection methods, including bootstrapping soft shrinkage approach (BOSS), Monte Carlo uninformative variable elimination (MCUVE), genetic algorithm partial least squares (GA-PLS) and competitive adaptive reweighted sampling (CARS) respectively.
  • The results showed that BOSS method obtained the lowest root mean squared error of cross validation (RMSECV) (0.0245) and root mean squared error of prediction (RMSEP) (0.0271), as well as the highest coefficient of determination of cross-validation (Q<sub>cv</sub><sup>2</sup>) (0.9998) and the coefficient of determination of test set (Q<sup>2</sup><sub>test</sub>) (0.9989), which demonstrated that the mid infrared spectroscopy can be used to detect Chlorantraniliprole in Abamectin conveniently.
  • Meanwhile, a suitable wavelength selection method (BOSS) is essential to conducting a component spectral analysis.

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  • [Copyright] Copyright © 2017. Published by Elsevier B.V.
  • (PMID = 29054068.001).
  • [ISSN] 1873-3557
  • [Journal-full-title] Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
  • [ISO-abbreviation] Spectrochim Acta A Mol Biomol Spectrosc
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] England
  • [Keywords] NOTNLM ; BOSS / Chlorantraniliprole / MIR / Wavelength selection
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10. Adolfsson K, Abariute L, Dabkowska AP, Schneider M, Häcker U, Prinz CN: Direct comparison between in vivo and in vitro microsized particle phagocytosis assays in Drosophila melanogaster. Toxicol In Vitro; 2018 Feb;46:213-218
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  • [Source] The source of this record is MEDLINE®, a database of the U.S. National Library of Medicine.
  • [Title] Direct comparison between in vivo and in vitro microsized particle phagocytosis assays in Drosophila melanogaster.
  • The effects of micro and nanoparticles on the innate immune system have been widely investigated and a general lack of agreement between in vivo and in vitro assays has been observed.
  • In order to determine the origin of these discrepancies, there is a need for comparing the results of in vivo and in vitro phagocytosis assays obtained using the same particles and same immune cells.
  • Here, we establish an in vivo polystyrene microsized particle phagocytosis assay in Drosophila melanogaster and compare it with an in vitro assay consisting of exposing the same immune cells in culture to the same particles.
  • The distribution of number of phagocytized beads per cell was shifted to lower numbers of beads per cell in the case of the in vitro assay compared to the in vivo assay, which we suggest is partly due to a reduced amount of membrane available in cultured cells.

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  • [Copyright] Copyright © 2017. Published by Elsevier Ltd.
  • (PMID = 29024778.001).
  • [ISSN] 1879-3177
  • [Journal-full-title] Toxicology in vitro : an international journal published in association with BIBRA
  • [ISO-abbreviation] Toxicol In Vitro
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] England
  • [Keywords] NOTNLM ; Drosophila melanogaster / Immune cells / Particle toxicology / Phagocytosis / Phagocytosis assay / Polystyrene beads
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